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df 18-1128-41 AD, 2001-06 • p1
data file
Liquid chromatography
ÄKTAFPLC
High-performance purification
of proteins
Fast and easy protein purification
method wizard for easy programming
comprehensive column library
automatic scouting for purification development and
optimization
short separation times with modern media
Reliability in every run
consistent reproducibility with high sensitivity
efficient fraction collection
automatic evaluation and report procedures
easy, fast scale-up
Flexibility for any purification
easy system modification
all chromatographic techniques
ÄKTAdesign support
Introduction
ÄKTA™FPLC™ is a biocompatible, high performance liquid
chromatography system for fast and easy purification of
proteins. The unique design provides a compact, ready to
use chromatography system (Fig 1) with an expandable rack
(Fig 2). The rack system gives built-in flexibility for the use
of longer chromatographic columns, changes in system
configuration, and increased automation.
ÄKTA
FPLC is controlled by UNICORN™, the control,
evaluation, and reporting platform for ÄKTA™design
systems, ensuring quick and simple communication between
systems and users. UNICORN control system is designed
for Windows NT™/Windows 2000 and includes an option
for network control of multiple systems.
ÄKTA
FPLC is compatible with all solvents commonly used in
protein purification, including the high salt concentrations
used in hydrophobic interaction chromatography.
Fig 1. ÄKTAFPLC system.
Fig 2. Rack system provides built-in flexibility.
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Inhaltsverzeichnis

Seite 1 - ÄKTAFPLC

df 18-1128-41 AD, 2001-06 • p1data fileLiquid chromatographyÄKTAFPLCHigh-performance purificationof proteinsFast and easy protein purification• met

Seite 2 - Reliability in every run

Liquid chromatographydf 18-1128-41 AD, 2001-06 • p10Examples of functionalities available for ÄKTAFPLC are listedbelow:On-line measurement of pH co

Seite 3 - Liquid chromatography

Liquid chromatographydf 18-1128-41 AD, 2001-06 • p11ÄKTAdesign supportÄKTAdesign knowledgeKnowledge built into the method wizard and columnknowledg

Seite 4 - • Affinity chromatography

Liquid chromatographydf 18-1128-41 AD, 2001-06 • p12System DescriptionGeneral descriptionÄKTAFPLC is supplied pre-assembled and tested (Fig 18). Th

Seite 5 - FPLC contains an

Liquid chromatographydf 18-1128-41 AD, 2001-06 • p13Figure 20 shows the simple and logical flow configuration ofa standard ÄKTAFPLC providing:• eas

Seite 6

Liquid chromatographydf 18-1128-41 AD, 2001-06 • p14Monitor UPC-900Monitor UPC-900 is a high precision on-line monitoroffering the unique possibili

Seite 7

Liquid chromatographydf 18-1128-41 AD, 2001-06 • p15With Frac-900 and flow diversion valve FV-903Tube capacity 95 in Tube Rack 18 mm175 in Tube Rac

Seite 8

df 18-1128-41 AD, 2001-06 • p16Liquid chromatographyOrdering informationProduct Code No.ÄKTAFPLC (254 and 280 nm detection, 18-6083-20conductivity

Seite 9 - FPLC components

Liquid chromatographydf 18-1128-41 AD, 2001-06 • p2Fast and easy protein purificationBegin work immediatelySee the real-time status of the runDirec

Seite 10 - • Recovery calculations

Liquid chromatographydf 18-1128-41 AD, 2001-06 • p3Flexibility for any protein purification3 00001 0002 0000 50 100 150 200 250020406080mAUmS/cmmlD

Seite 11 - ÄKTAdesign support

Liquid chromatographydf 18-1128-41 AD, 2001-06 • p4Figure 5 and 6 shows how fast you can start an existingmethod and the simplicity of programming

Seite 12 - System Description

Liquid chromatographydf 18-1128-41 AD, 2001-06 • p5– comprehensive column libraryFor simple, safe column selection ÄKTAFPLC contains anextensive co

Seite 13 - Flow configuration

Liquid chromatographydf 18-1128-41 AD, 2001-06 • p6– short separation times with modern mediaÄKTAFPLC provides fast and reliable protein purificati

Seite 14 - System specifications

Liquid chromatographydf 18-1128-41 AD, 2001-06 • p7Selection of the correct wavelength for detection of the targetprotein is essential to achieve h

Seite 15 - Environment

Liquid chromatographydf 18-1128-41 AD, 2001-06 • p8– automatic evaluation and report proceduresExtensive data evaluation is available. For automati

Seite 16 - Ordering information

Liquid chromatographydf 18-1128-41 AD, 2001-06 • p9Fig 15. Additional equipment is easily added to an ÄKTAFPLC system.ÄKTAFPLC configuration with o

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