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df 18-1119-48 AC, 2001-08 • p1
datafile
Liquid chromatography
ÄKTApurifier chromatography systems
High-performance purification
and characterization of proteins,
peptides and nucleic acids
Fast and easy start to purification
method wizard for easy programming
Speed and reliability for every run
fast optimization and peak identification, accurate result
comparisons, improved peak purity, minimal procedure times
with column knowledge built-in
Increased reproducibility every day
automatic buffer preparation, evaluation and report
procedures
Flexibility to change with your needs
easy system modification
increased productivity and throughput
ÄKTAdesign support
Introduction
ÄKTA™purifier (Fig 1) is a liquid chromatography system
designed for fast and reliable separations of proteins,
peptides and nucleic acids.
ÄKTApurifier runs all chromatography techniques, from µg
to mg scale at flow rates 0.001 ml/min – 10 ml/min and
pressures 0–25 MPa or alternatively at flow rates 0.01–100
ml/min and pressures 0–10 MPa. With operational simplicity
and the method wizard ÄKTApurifier incorporates many
features to minimize preparation and run times and
maximize reliability and reproducibility of results.
ÄKTApurifier is essential to guarantee fast, reproducible
runs everyday, as shown in Figure 2 and Figure 3.
Fig 1. ÄKTApurifier, from the ÄKTApurifier chromatography
system series.
ÄKTApurifier systems are part of ÄKTAdesign, a range of
liquid chromatography systems for academic and industrial
research laboratories. ÄKTAdesign systems are controlled
by UNICORN™ , a control, evaluation and reporting
platform which also controls production systems at
kilogram scale. Designed for Windows NT™/Windows
2000, UNICORN ensures quick, simple communication
between systems and users and meets the stringent control
and data handling procedures of modern laboratories.
Optimized methods are transferred easily to production
scale.
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Inhaltsverzeichnis

Seite 1 - • easy system modification

df 18-1119-48 AC, 2001-08 • p1datafileLiquid chromatographyÄKTApurifier chromatography systemsHigh-performance purificationand characterization of

Seite 2

Liquid chromatographydf 18-1119-48 AC, 2001-08 • p10Share dataServerPC NetworkRoom 1ÄKTAexplorer 100 AÄKTAFPLC™ÄKTApurifier 10 XTÄKTAexplorer 10 XT

Seite 3 - Experimental conditions

Liquid chromatographydf 18-1119-48 AC, 2001-08 • p11ÄKTAdesign service• Controlled costs• Traceable results• Regulatory requirements support• Maxim

Seite 4

Liquid chromatographydf 18-1119-48 AC, 2001-08 • p12Figure 23 shows the configuration of ÄKTApurifierchromatography systems providing:• easy pump w

Seite 5

Liquid chromatographydf 18-1119-48 AC, 2001-08 • p13Both pumps can be used for loading samples larger than50 ml, but for easy, routine handling of

Seite 6

Liquid chromatographydf 18-1119-48 AC, 2001-08 • p14Operating dataP-903Flow rate range 0.001–10 ml/minIncrement 0.001 ml/minPressure range 0–25 MPa

Seite 7

Liquid chromatographydf 18-1119-48 AC, 2001-08 • p15Sample Pump P-950Flow rate range 0.1–50 ml/minOperating pressure 0–1.0 MPaViscosity 5 cP10 cP a

Seite 8

df 18-1119-48 AC, 2001-08 • p16Liquid chromatographyProduced by Wikströms, Sweden 1010980, 08.2001Printed matter. Licence 341 051Ordering informati

Seite 9 - • Recovery calculations

Liquid chromatographydf 18-1119-48 AC, 2001-08 • p2002202Time (min)10050%cond.05mAUBufferPrep modeProtein mixtureRESOURCE Q10 ml/minpH 5.0pH 6.5 pH

Seite 10 - ÄKTAdesign support

Liquid chromatographydf 18-1119-48 AC, 2001-08 • p34003002001000mS/cm1.21.00.80.60.0 10.0 20.0 30.0 mlmAUYADKITEDLK30.020.010.00.00mS/cm3.02.52.01.

Seite 11 - System description

Liquid chromatographydf 18-1119-48 AC, 2001-08 • p4Fast and easy start to purificationUsers are able to create complete purification schemes for ar

Seite 12 - System components

Liquid chromatographydf 18-1119-48 AC, 2001-08 • p5Speed and reliability for every runRoutine runs quickly optimizedTo quickly establish fast routi

Seite 13 - Optional

Liquid chromatographydf 18-1119-48 AC, 2001-08 • p6 Improved peak purityAccurate peak fractionation can help to improve the purityof a selected pea

Seite 14 - System specifications

Liquid chromatographydf 18-1119-48 AC, 2001-08 • p7The selection of the correct column for each purification stepis essential for optimum results.

Seite 15 - Conductivity unit

Liquid chromatographydf 18-1119-48 AC, 2001-08 • p8Increased reliability and reproducibilityfrom day to dayAccurate, automatic buffer preparationTh

Seite 16 - Liquid chromatography

Liquid chromatographydf 18-1119-48 AC, 2001-08 • p9Fig 16. BufferPrep for automatic, rapid selection of pH.2502001501005002.0mAU280 nmmS/cm80.060.0

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